Translational_Unit
rbs+sam5

Part:BBa_I742147:Design

Designed by: sarah hollingshead   Group: iGEM07_Edinburgh   (2007-10-24)


sam5 (coumarate hydroxylase) with native rbs.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1436
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1436
    Illegal NgoMIV site found at 37
    Illegal NgoMIV site found at 45
    Illegal NgoMIV site found at 795
    Illegal NgoMIV site found at 968
    Illegal NgoMIV site found at 1370
    Illegal NgoMIV site found at 1374
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 569


Design Notes

This was made as a single biobrick, rather than separate rbs and coding sequence, hence the use of blunt ligation in generating this composite part.


Source

Saccharothrix espanaensis DSM 44229

References